Specific binding of glucosaminylmuramyl peptides to histones

Co-operative binding of histones to DNA.

The interaction of certain components of chromatin was studied in a model system. It was found that when calf thymus histones were mixed with sonicated phage DNA and the macromolecules made to interact by lowering the ionic strength, a mixture of complexes was formed, each separable by velocity sedimentation and characterized by unique thermal stability properties and histone contents. The bind...

Identification, Characterization and Utility of Prostate-specific Antigen- Binding Peptides

Interactions of Hsp90 with histones and related peptides.

The 90 kDa heat shock protein (Hsp90) induces the condensation of the chromatin structure [Csermely, P., Kajtár, J., Hollósi, M., Oikarinen, J., and Somogyi, J. (1994) Biochem. Biophys. Res. Commun. 202, 1657-1663]. In our present studies we used surface plasmon resonance measurements to demonstrate that Hsp90 binds histones H1, H2A, H2B, H3 and H4 with high affinity having dissociation constan...

Promiscuous and allele-specific anchors in HLA-DR-binding peptides.

The major histocompatibility complex (MHC) class II molecules are highly polymorphic membrane glycoproteins that bind peptide fragments of proteins and display them for recognition by CD4+ T cells. To understand the effect of human MHC class II polymorphism on peptide-MHC interaction, we have isolated M13 phage from a large M13 peptide display library by selection with DRB1*0401 and DRB1*1101 m...

N-Terminal acetyl-peptides from two calf thymus histones.

1. The same N-terminal peptide was isolated from two major calf thymus histone fractions, F2al and F2a2. 2. The structure of the peptides is N-acetylseryl-glycyl-arginine, and the yields account for most of the N-acetyl groups previously found in these histones. 3. Some other peptides not giving a ninhydrin reaction were also investigated.